目前我在培养hFOB1.19细胞系，由于细胞库买不到，就经人介绍从细胞公司购买了，前后发了3次才自己培养活，按照说明条件进行培养，目前细胞贴壁状态不是很好，由于实验室条件限制只能在36.5℃培养（无法在33.5℃下培养），请问有没有人用此细胞系做过实验，想请教一下：1、培养温度是否对细胞影响大？2、我的培养液配比：F12 DMEM90%+Gibco胎牛血清10%+双抗+0.3g/ml G418，是否有优化（我曾增加了血清浓度，改变不大）的空间？3、这个细胞培养几天翻一倍？多久换一次液较好？4、6cm或15cm dish培养时铺盘时如何种最好？或者说长满后1传几长得比较好？谢谢各位路过的大神给予解答。
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10 branched poly(Arg, epsilon-Lys)-hyaluronic acid hydrogel (for 3D cell culture)
Xenogenic-free, chemically defined, highly reproducible and excellent biocompatibility
Without crosslinker and organic solution
Mimic the natural extracellular matrix(ECM).
Proper mechanical strength
Storage at room temperature
No need to pre-treat prior to using with cell suspension
Mesh size 50, 100, 150μm
83USD/10 pills
Xenogenic-free, chemically defined, highly reproducible and excellent biocompatibility
Without crosslinker and organic solution
Mimic the natural extracellular matrix(ECM).
Proper mechanical strength
Storage at room temperature
No need to pre-treat prior to using with cell suspension
Mesh size 50, 100, 150μm
83USD/10 pills
